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MEASUREMENT METHODOLOGY

Antisera for various animal a1AG were produced in rabbits. These reagents permit the quantitative measurement of a1AG levels from serum by means of an immunodiffusion method, an enzyme immunoassay or a latex agglutination method. The immunodiffusion  method or SRID has been shown to be exceedingly reproducible and accurate. Further, the SRID method has been easier to perform than G-IEF, rocket immunoelectrophoresis, ELISA or radloimmunoassay.

With the SRID method, 5m1 of a specimen is placed into a small well of an agar plate which contains a1AG antiserum. The plate is incubated for 24 to 48 hours at 370C. The specific amount of a1AG within the specimen is measured by the size of the manifest precipitin ring and is determined by comparison to values for a high and low standard provided with each test kit.

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